The Cyr61 Is a Potential Target for Rotundifuran, a Natural Labdane-Type Diterpene from Vitex trifolia L., to Trigger Apoptosis of Cervical Cancer Cells

Figure 1

RTF suppressed the proliferation of cervical cancer cell lines of HeLa and SiHa via induction of apoptosis. (a) Cytotoxic activities of RTF on cervical cancer cell lines of HeLa and SiHa. Cell were treated with RTF for 24 or 48 h, and then, the CCK-8 assay was used to detect the cell proliferation inhibition (%), and IC₅₀ values of RTF against HeLa and SiHa cells were calculated. (b) Apoptotic assay by DAPI staining. Cells were treated with RTF for 24 hours, and then, the apoptotic cells were detected by DAPI staining and visualized under a fluorescent microscope (×200). (c, d) Cell cycle arrest and apoptosis detection by flow cytometry. Cells were treated with RTF for 24 h, and the apoptotic cells were detected by staining with PI and Annexin V-FITC/PI followed by flow cytometry analysis, respectively. Data are expressed as , and the asterisks indicate significant difference, , vs. Control.

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