The Cyr61 Is a Potential Target for Rotundifuran, a Natural Labdane-Type Diterpene from Vitex trifolia L., to Trigger Apoptosis of Cervical Cancer Cells

Figure 2

RTF induced increased ROS level and reduced MMP in cervical cancer cells. (a, c) ROS level determination of HeLa and SiHa cells. Cells were treated with RTF for 24 h, and then, the ROS level was detected by staining with a DCFH-DA fluorescent probe and visualized under a fluorescent microscope (×200) and flow cytometry. (b) MMP determination of HeLa and SiHa cells. Cells were treated with RTF for 24 h, and then, the ROS level was detected by staining with a JC-1 fluorescent probe and visualized under a fluorescent microscope (×200); H₂O₂ (20 μM) was used as the positive control.

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