The Cyr61 Is a Potential Target for Rotundifuran, a Natural Labdane-Type Diterpene from Vitex trifolia L., to Trigger Apoptosis of Cervical Cancer Cells

Figure 6

Cyr61 was the drug target of RTF for treating cervical cancers. DARTS (a) and CETSA (b) analyses. Cells were exposed to RTF and 0.5% DMSO for 3 h, and total proteins were extracted and treated with pronase and heat; finally, the reaction products were analyzed using western blotting assays. (c) MST assay. The labeled protein samples were mixed with serial dilutions of RTF samples, and the mixture was loaded into standard capillaries and scanned by an MST instrument. Finally, the value was determined using NanoTemper Analysis 2.3 software. (d) Molecular docking assay. Molecular docking was performed using the Glide 6.9 module of Schrödinger software.

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