PGC-1α Protects against Hepatic Ischemia Reperfusion Injury by Activating PPARα and PPARγ and Regulating ROS Production

Figure 1

Hepatic I/R enhances the in situ expression of PGC-1α, and transduction with adenoviral (Ad) vectors is effective for inducing intrahepatic PGC-1α overexpression or knockdown. (a) The mRNA expression of PGC-1α in the mouse liver of each group was assessed by real-time RT-PCR (). (b) The protein expression of PGC-1α was detected by western blot. (c) Fluorescent microscopy showed the efficient transduction of hepatocytes, as indicated by GFP expression, 72 h following administration of the Ad vectors. (d) PGC-1α protein expression in the mouse liver of each group was assessed by western blot analysis. Bar: 100 μm. , , and . The results are representative of three independent experiments.

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