PGC-1α Protects against Hepatic Ischemia Reperfusion Injury by Activating PPARα and PPARγ and Regulating ROS Production

Figure 3

PGC-1α regulates the activation of PPARα and PPARγ in vivo and in vitro and protects A/R-induced hepatocyte injury. (a) The activation of PPARα and PPARγ in liver tissues was assessed in the mice subjected to Ad-PGC-1α, Ad-shPGC-1α, and the related control at 6 h after liver I/R by EMSA (A), and the specificity of the PPARα and PPARγ bands were confirmed with competition and supershift assays; the supershift bands are indicated by the arrow (B). (b) Representative fluorescence microscopy images of the hepatocytes from certain groups were taken at 24 h after A/R injury. (c) Cell viability was determined at 24 h after reoxygenation by a CCK-8 assay. (d) DNA fragmentation was determined at 24 h after reoxygenation. (e) LDH release was measured in each group. (f) The activation of PPARα and PPARγ in the hepatocytes was assessed by EMSA (A), and the specificity of the PPAR α and γ bands was also confirmed (B). The data was presented as the of three independent experiments. Bar: 200 μm, , , and . NE: nuclear extracts; SC: specific competition; NSC: nonspecific competition; anti-PPARα: PPARα antibody; anti-PPARγ: PPARγ antibody.

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