PGC-1α Protects against Hepatic Ischemia Reperfusion Injury by Activating PPARα and PPARγ and Regulating ROS Production

Figure 4

Inhibition of either PPARα or PPARγ alleviates the hepatoprotective effect of PGC-1α in vivo. (a) The serum levels of aminotransferases were measured in the mice subjected to Ad-PGC-1α, Ad-PGC-1α+MK886, Ad-PGC-1α+GW9662, and Ad-PGC-1α+MK886+GW9662 at 6 h after liver I/R (). (b) Representative images (200x magnification) of the H&E-stained liver sections (6 h after I/R) were taken, and histopathological scoring of hepatic injury was performed (). (c) Representative images (200x magnification) of the liver sections (6 h after I/R) stained by TUNEL were taken, and TUNEL-positive cells were counted as described in Materials and Methods (). (d–f) Caspase-3 activity, DNA fragmentation, and cleaved PARP expression in the mouse livers of each group were assessed by ELISA and western blot analysis at 6 h after I/R (-6). , , and .

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