PGC-1α Protects against Hepatic Ischemia Reperfusion Injury by Activating PPARα and PPARγ and Regulating ROS Production

Figure 6

PGC-1α regulates the metabolism of ROS in hepatocytes in vivo and in vitro and decreases liver I/R-induced damage of oxidative stress. (a) The representative images of DHE-stained liver cryosections from mice subjected to Ad-GFP, Ad-PGC-1α, Ad-shScramble, and Ad-shPGC-1α at 1 h after liver I/R and the relative ROS levels. Bar: 200 μm (). (b) Representative images of hepatocytes stained by DHE, and the relative ROS levels. Bar: 200 μm (). (c) The hepatic content of MDA at 6 h after liver I/R (). (d) The hepatic content of HNE at 6 h after liver I/R (). (e) The hepatic content of MDA from mice subjected to Ad-PGC-1α, Ad-PGC-1α+MK886, Ad-PGC-1α+GW9662, and Ad-PGC-1α+MK886+GW9662 at 6 h after liver I/R (). (f) The hepatic content of HNE in the groups. , , and .

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