Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression

Figure 2

Effects of PBMT on the loss of astrocytes and expression of GLT-1 in the cortex and hippocampus of depressed mice. (a) Representative immunofluorescent images of GFAP in the hippocampal and cortex regions of each group. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm ( per group). (b) Representative immunofluorescent images of GLT-1 in the hippocampal and cortex regions of each group. Scale bar: 100 μm ( per group). (c) Quantification analysis of GFAP-positive cells in the hippocampal and cortex regions of different groups. (d) Quantification analysis of GLT-1 in the hippocampal and cortex regions of different groups. (e–h) GFAP and GLT-1 expression detected by western blot in the cortex and hippocampus lysates from mice exposed to CUMS with or without PBMT ( mice for each group). Data represent . ns: not significant. , , and , one-way ANOVA with Tukey’s post hoc analysis. DAPI: 4,6-diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; GLT-1: glutamate transporter-1.

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