PAR2 Deficiency Induces Mitochondrial ROS Generation and Dysfunctions, Leading to the Inhibition of Adipocyte Differentiation

Figure 6

PAR2 deficiency reduces calcium signaling in preadipocytes. Preadipocytes were isolated from sWAT of male WT and PAR2 KO mice (aged 5 weeks). (a) SLIGRL-NH₂-mediated calcium mobilization by activation of PAR2. Representative images of western blot analysis show protein levels of (b) CaM and p-CaMKII (cytosol), (c) p-CREB and PGC-1α (nucleus), (d) p-AMPK (cytosol), and (e) TFAM (whole lysate) in the cells. (f) Preadipocytes were treated with the PAR2 agonist (SLIGRL-NH₂) or antagonist (GB83) for 24 h, and fluorescent microscopic analysis was performed. (g) MitoTracker staining images were quantified by ImageJ (). Data are expressed as the . and relative to control.

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