Research Article
Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe
Figure 4
Determination of the optimal conditions for detection of the RB700 signal using the IVIS™ imaging system. RB700 (1-100 μM) was oxidized in the presence of Fenton’s reagent (10 μM μM H2O2) for 1 minute. (a) A representative image of epifluorescent emission is shown in panel. (b) Quantitative detection of the signal generated by the increasing concentrations of oxidized RB700. , . Statistical values were calculated relative to the level of fluorescence associated with the 1 μM of oxidized RB700. (c) Spectral analysis by removing the background signal that highlights the specificity of the RB700 fluorescence emission in the near-infrared range. (d) Representative image demonstrating the absence of RB700 emission in the green spectral range is shown in panel.
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