Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe

Figure 8

Colocalization of ROS-induced RB700 oxidation with the advanced plaque in the atherosclerotic aorta of ApoE-/- mice. C57BL/6J mice (controls, normal diet/ND, 10 weeks), ApoE-/- mice (ND, 10 weeks), and ApoE-/- that received high-cholesterol diet (HD) for 32 weeks were injected retroorbital with VLp-RB700, and after 2 hours, the animals were sacrificed, and the aortas collected and analyzed using the IVIS™ system. (a, b) Quantitative analysis of epifluorescent emission throughout the aorta of experimental mice. (c) The colocalization of the oxidized RB700 fluorescence signal after spectral unmixing at the level of atherosclerotic lesions (arrows) present along the aorta of ApoE-/- mice fed with the atherogenic diet for 32 weeks is observed. , , . The statistical value was calculated in relation to the C57BL6/J (ND) level.

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