Research Article
Polydatin Attenuates OGD/R-Induced Neuronal Injury and Spinal Cord Ischemia/Reperfusion Injury by Protecting Mitochondrial Function via Nrf2/ARE Signaling Pathway
Figure 3
PD attenuates OGD/R-induced damage in SMNs. Cells were divided into the Control, PD (15 μM), PD (30 μM), OGD/R, OGD/R + PD (15 μM), and OGD/R + PD (30 μM) groups. (a) Representative images showing Annexin V-FITC/PI stained cells. (b) Representative images showing TUNEL-positive cells. μm. Quantification of the apoptotic cells in (c) Annexin V-FITC/PI and (d) TUNEL assays. (e) Immunoblot showing Bcl-2, Bax, and c-Caspase-3 protein levels in the neurons of the indicated groups. (f–h) Quantification of the relative protein levels. vs. Control; # vs. OGD; Δ vs. OGD + PD (15 μM).
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