Inhibition of Inducible Nitric Oxide Synthase (iNOS) by Andrographolide and In Vitro Evaluation of Its Antiproliferative and Proapoptotic Effects on Cervical Cancer

Figure 9

Flow cytometric analysis of andrographolide-treated HeLa cells showed dose-dependent (a–d) cell cycle arrest at G1/S transition phase, (e–h) Annexin V-FITC/PI-stained apoptotic cells. (a, e) Represent untreated control, (b, f) represent 5 μM of andrographolide, and (c, g) represent 10 μM of andrographolide. (d) Relative distribution of cell cycle percentage and (h) comparative percentage of the cell population in early and late apoptosis in control and andrographolide treatments.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)