Research Article

Effects of Fluorine on Neutrophil Extracellular Trap Formation through Regulating AMPK/p38 Signaling Pathway

Figure 3

NaF induced ROS production. Neutrophils were seeded into the 96-well plates and treated with DCFH-DA staining solution for 30 min. After washing with RPMI 1640 medium (phenol-red free), the cells were treated with NaF (0.25, 0.5, and 1 mM) for 2 h. Zymosan was used as the positive control group. The data were presented as and statistical significance is expressed as and .