Promoting Nrf2/Sirt3-Dependent Mitophagy Suppresses Apoptosis in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration

<div>t-BHQ alleviates the imbalance of mitochondrial dynamics induced by TBHP and promotes mitophagy in a NRF2/SIRT3 pathway-dependent manner. (a, b) Successful knockdown of Sirt3. (c, d) The protein expression of LC3, P62, NRF2, histone H3, SIRT3, and cleaved caspase-3 in NPCs. (e) The promoter of Sirt3 was ChIP-ed with anti-Nrf2 antibody or IgG control. (f) Immunofluorescence double-labeled staining for colocalization of LC3 with TOM20 in NPCs. (g–j) TUNEL, MitoTracker, and MitoSOX staining assays were performed in NPCs. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Promoting Nrf2/Sirt3-Dependent Mitophagy Suppresses Apoptosis in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration 

Figure 6 | Promoting Nrf2/Sirt3-Dependent Mitophagy Suppresses Apoptosis in Nucleus Pulposus Cells and Protects against Intervertebral Disc Degeneration 