Brusatol Inhibits Tumor Growth and Increases the Efficacy of Cabergoline against Pituitary Adenomas

Figure 3

Brusatol- (BT-) induced apoptotic cell death in GH3 and MMQ cells. (a, b) Induction of apoptosis in GH3 and MMQ cells with BT treatment (250 nM) for 24 h followed by Annexin V and PI staining. Apoptosis ratios were measured by flow cytometry. (c, d) After 0–24 h treatment with BT (250 nM) in GH3 and MMQ cells, Western blot analysis was used to monitor the expression level of c-caspase-3, c-caspase-8, and Bcl-2. (e, f) GH3 and MMQ cells were treated with BT or NAC (100 μM) alone or in combination for 24 h and MTS assays conducted to determine cell viability. Data are represented as the . .

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