Research Article
Deferasirox, an Iron-Chelating Agent, Improves Testicular Morphometric and Sperm Functional Parameters in a Rat Model of Varicocele
Table 1
List of primers used for real-time RT-PCR analysis.
| | Gene | Primer sequence (5-3) | GenBank no. | Amplicon size (bp) |
| | Grp78 | F TAACAATCAAGGTCTACGAAGG
R CCATTCACATCTATCTCAAAGGT | NM_013083.2 | 193 | | Splice xbp-1 | F CTGAGTCCGCAGCAGG
R CTTGTCCAGAATGCCCAAAAGG | NM_001271731.1 | 119 | | Unspliced xbp-1 | F GTCCGCAGCACTCAGACTAC
R CTGGGGAAGGACATTTGAAAAAC | NM_001004210.2 | 178 | | Nrf2 | F TGCCATTAGTCAGTCGCTC
R GTGCCTTCAGTGTGCTTC | NM_031789.2 | 99 | | Bcl-2 | F ACTTCTCTCGTCGCTACCGTC
R AAGAGTTCCTCCACCACCGT | NM_016993.1 | 106 | | Bax | F GGATCGAGCAGAGAGGATGG
R ACACTCGCTCAGCTTCTTGG | NM_017059.2 | 91 | | Bak | F CAGAGAGGTGGTTGGGTGG
R GTGGGTTGGGGAGAGGTTTAG | NM_053812.1 | 181 | | Bim | F CACAAACCCCAAGTCCTCC
R AGTCTCATTGAACTCGTCTCC | NM_171988.2 | 152 | | Caspase-3 | F CGGTATTGAGACAGACAGTGGAAC
R GCGGTAGAGTAAGCATACAGGAAG | NM_012922.2 | 90 | | p53 | F CCGACTATACCACTATCCACTAC
R CACAAACACGAACCTCAAAGC | NM_030989.3 | 147 | | GAPDH | F TGCCGCCTGGAGAAACC
R TGAAGTCGCAGGAGACAACC | NM_017008.4 | 121 |
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