Research Article
Coronary Endothelium No-Reflow Injury Is Associated with ROS-Modified Mitochondrial Fission through the JNK-Drp1 Signaling Pathway
Figure 1
Hypoxia/reoxygenation injury mediates endothelial cell oxidative stress. (a) Cell viability was measured using the CCK-8 assay. Cardiac endothelial cells (CECs) underwent hypoxia/reoxygenation (H/R) injury. (b–e) The content of antioxidative factors was measured using an enzyme-linked immunosorbent assay (ELISA). (f, g) Intracellular ROS and mitochondrial ROS (mROS) were detected using DCFHDA and MitoSOX red mitochondrial superoxide indicator, respectively. .
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