H9c2 Cardiomyocytes under Hypoxic Stress: Biological Effects Mediated by Sentinel Downstream Targets

Figure 3

Western blotting analysis for RhoA, Rac1, myogenin, and MyoD in normalized lysates. Lysate proteins (1 mg/ml) were resolved by electrophoresis and analyzed by Western blot, using the antibodies against the indicated proteins (a). The band intensity was determined by densitometric analysis using the ImageJ software (b, c).