Increased ROS-Dependent Fission of Mitochondria Causes Abnormal Morphology of the Cell Powerhouses in a Murine Model of Amyotrophic Lateral Sclerosis

Table 1

Primary and secondary antibodies used for Western blotting.

Antibody	Dilution	Order number
Anti-MFN1 mouse monoclonal IgG antibody	1 : 100 in Roti-TBS (#1060.1, Roth, Germany)	#sc-166644, Santa Cruz, USA
Anti-MFN2 mouse polyclonal IgG antibody	1 : 100 in Roti-TBS (#1060.1, Roth, Germany)	#ARP89255_P050, Aviva systems biology, USA
Anti-OPA1 mouse monoclonal IgG antibody	1 : 100 in Roti-TBS (#1060.1, Roth, Germany)	#sc-393296, Santa Cruz, USA
Anti-OMA1 mouse monoclonal IgG antibody	1 : 100 in Roti-TBS (#1060.1, Roth, Germany)	#sc-515788, Santa Cruz, USA
Anti-Drp1 mouse monoclonal IgG antibody	1 : 500 in Roti-TBS-T (#1061.1, Roth, Germany)	#14647, Cell Signaling, USA
Anti-pDrp1 rabbit monoclonal IgG antibody	1 : 500 in Roti-TBS-T (#1061.1, Roth, Germany)	#4494, Cell Signaling, USA
Anti-CaMKII (pan) rabbit polyclonal antibody	1 : 500 in Roti-TBS-T (#1061.1, Roth, Germany)	#3362, Cell Signaling, USA
Anti-ox-CaMKII rabbit polyclonal antibody	1 : 500 in Roti-TBS-T (#1061.1, Roth, Germany)	#07-1387 Merck, Germany
Anti-calnexin rabbit polyclonal IgG antibody	1 : 200 in Roti-TBS (#1060.1, Roth, Germany)	#sc-11397, Santa Cruz, USA
Anti-actin rabbit polyclonal IgG antibody	1 : 1000 in Roti-TBS (#1060.1, Roth, Germany)	#A2668; Merck, Germany
Anti-rabbit goat horseradish-peroxidase-conjugated antibody	1 : 10.000 in Roti-TBS (#1060.1, Roth, Germany)	#sc-2054, Santa Cruz, USA
Anti-mouse donkey antibody	1 : 10.000 in Roti-TBS (#1060.1, Roth, Germany)	#sc-2314, Santa Cruz, USA