Thermal Stability Kinetics and Shelf Life Estimation of the Redox-Active Therapeutic and Mimic of Superoxide Dismutase Enzyme, Mn(III) meso-Tetrakis(N-ethylpyridinium-2-yl)porphyrin Chloride (MnTE-2-PyPCl5, BMX-010)

Maia, Clarissa G. C.; de Araujo, Bárbara C. R.; de Freitas-Marques, Maria B.; da Costa, Israel F.; Yoshida, Maria Irene; da Nova Mussel, Wagner; Sebastião, Rita de Cássia O.; Rebouças, Júlio S.

doi:https://doi.org/10.1155/2021/7003861

Oxidative Medicine and Cellular Longevity

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

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Mimics of Superoxide Dismutase Enzymes and Other Redox-Active Compounds as Gatekeepers of the Cellular Redox Environment

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Volume 2021 | Article ID 7003861 | https://doi.org/10.1155/2021/7003861

Thermal Stability Kinetics and Shelf Life Estimation of the Redox-Active Therapeutic and Mimic of Superoxide Dismutase Enzyme, Mn(III) meso-Tetrakis(N-ethylpyridinium-2-yl)porphyrin Chloride (MnTE-2-PyPCl₅, BMX-010)

Clarissa G. C. Maia,¹Bárbara C. R. de Araujo,²Maria B. de Freitas-Marques,²Israel F. da Costa,¹Maria Irene Yoshida,²Wagner da Nova Mussel,²Rita de Cássia O. Sebastião,²and Júlio S. Rebouças¹

Academic Editor: Grzegorz Wegrzyn

Received02 Jun 2021

Accepted12 Nov 2021

Published06 Dec 2021

Abstract

Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin chloride (MnTE-2-PyPCl₅, BMX-010, and AEOL10113) is among the most studied superoxide dismutase (SOD) mimics and redox-active therapeutics, being currently tested as a drug candidate in a phase II clinical trial on atopic dermatitis and itch. The thermal stability of active pharmaceutical ingredients (API) is useful for estimating the expiration date and shelf life of pharmaceutical products under various storage and handling conditions. The thermal decomposition and kinetic parameters of MnTE-2-PyPCl₅ were determined by thermogravimetry (TG) under nonisothermal and isothermal conditions. The first thermal degradation pathway affecting Mn-porphyrin structural integrity and, thus, activity and bioavailability was associated with loss of ethyl chloride via N-dealkylation reaction. The thermal stability kinetics of the N-dealkylation process leading to MnTE-2-PyPCl₅ decomposition was investigated by using isoconversional models and artificial neural network. The new multilayer perceptron (MLP) artificial neural network approach allowed the simultaneous study of ten solid-state kinetic models and showed that MnTE-2-PyPCl₅ degradation is better explained by a combination of various mechanisms, with major contributions from the contraction models R1 and R2. The calculated activation energy values from isothermal and nonisothermal data were about 90 kJ mol^–1 on average and agreed with one another. According to the R1 modelling of the isothermal decomposition data, the estimated shelf life value for 10% decomposition () of MnTE-2-PyPCl₅ at 25°C was approximately 17 years, which is consistent with the high solid-state stability of the compound. These results represent the first study on the solid-state decomposition kinetics of Mn(III) 2-N-alkylpyridylporphyrins, contributing to the development of this class of redox-active therapeutics and SOD mimics and providing supporting data to protocols on purification, handling, storage, formulation, expiration date, and general use of these compounds.

1. Introduction

Water-soluble, cationic Mn(III) porphyrins derived from the 2-N-pyridylporphyrin scaffold were originally developed as potent superoxide dismutase (SOD) mimics, peroxynitrite scavengers, and later proven to be efficient redox-active therapeutics [1–6]. Two lead compounds, Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin chloride (MnTE-2-PyPCl₅, also known as BMX-010) [2] and Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin chloride (MnTnBuOE-2-PyPCl₅, also known as BMX-001) [7, 8], were found to be safe and well tolerated in phase I clinical trials [1, 9–13]. Whereas MnTE-2-PyPCl₅ progressed to various phase II clinical trials on atopic dermatitis and itch, the MnTnBuOE-2-PyPCl₅ analogue, which was developed based on the MnTE-2-PyPCl₅ prototype, is now being investigated in four phase II clinical trials as radioprotectors for glioma, head and neck cancer, multiple brain metastases, and anal squamous cell carcinoma [1, 10, 11, 14].

MnTE-2-PyPCl₅ has an established reputation of being the Mn(III) 2-N-alkylpyridylporphyin-based prototype for the design of bioavailable SOD mimics, the development of catalytic antioxidants, and mechanistic studies [1, 3, 12, 15–17]. Given its safe toxicity profile in animal models and humans [1, 9, 10, 13], MnTE-2-PyPCl₅ is usually the compound of choice in most exploratory preclinical studies [12, 17–25], being forwarded to translational medicine and clinical trials [1, 9, 10], and is now recognized as an excellent redox-active therapeutic [1], with well-defined pharmacokinetics [26, 27]. Despite the large number of studies dedicated to unravelling the biological and clinical aspects of MnTE-2-PyPCl₅ both in vitro and in vivo [1, 16–18, 22], studies on the stability of this class of compounds are still somewhat limited [28–30]. It is worth noting, however, that information on MnTE-2-PyPCl₅ stability may shed light on the stability of other MnP analogues [28] and likely support protocols on purification, handling, storage, formulation, expiration date, and general use of these compounds [31–34].

As a characteristic of Mn-porphyrins in general, MnTE-2-PyPCl₅ with Mn in the +3 oxidation state is extremely stable against acid solvolysis: Mn(III) demetallation is difficult even under concentrate H₂SO₄ conditions [2]. MnTE-2-PyPCl₅ is relatively stable against oxidative degradation under biological conditions, particularly the presence of many reactive substrates or biological sacrificial reductors [35]. Not only do Mn-porphyrins withstand major reactive oxygen and nitrogen species (ROS/RNS) but also H₂O₂ is a key molecule in the therapeutic effects of Mn-porphyrins, as clearly shown by Jaramillo et al. [36] and recently summarized elsewhere [1]. Whereas MnTE-2-PyPCl₅ undergoes some bimolecular degradation when incubated with net H₂O₂ [22, 29], decomposition by peroxides is not at all an issue for solid-state/aqueous solution storage and/or general formulation of this compound [28]. The thermal degradation of MnTE-2-PyPCl₅, as a representative of the Mn(III) 2-N-alkylpyridylporphyrin class, has been addressed in a single thermogravimetric study so far [28]. The thermal behavior of MnTE-2-PyPCl₅ salts under aerobic conditions is characterized by three events: (i) dehydration of associated water molecules (room temperature to 134°C), (ii) N-dealkylation via loss of ethyl chloride (134–279°C) to yield MnT-2-PyPCl (see Figure 1), and (iii) various overlapping porphyrin-ring decomposition processes to yield manganese oxides as combustion residues (279–950°C) [28]. As MnTE-2-PyPCl₅ is often administered as aqueous formulations by various routes (e.g., intraperitoneal, subcutaneous, intravenous, and oral) [17, 26, 27], MnP intrinsic biological activity on the Mn basis is not affected by reversible dehydration [28]. Conversely, N-dealkylation of MnTE-2-PyPCl₅ changes the porphyrin structure irreversibly, reducing the overall charge of the molecule, which affects Mn(III)/Mn(II) redox potential, electrostatic facilitation for superoxide/peroxynitrite approach/scavenging, reactions with protein thiolates, and MnTE-2-PyP⁵⁺(aq) pharmacological activity [1, 17, 37, 38].

Thermogravimetric (TG) analyses are important for the evaluation and/or comparison of thermal stabilities of pharmaceutical materials, the study of drug-excipient compatibility in drug products, and the determination of kinetic parameters associated with thermal processes (such as activation energies, frequency factor, and reaction order) [31–34, 39, 40]. Overall, this information is useful for the development of pharmaceutical products and quality control [32, 34, 39, 41]. Studies on the kinetics of thermal degradation are recommended to evaluate the thermal stability of active pharmaceutical ingredients (API) and/or their solid-state pharmaceutic formulations [31–34, 39, 41, 42]. These kinetic studies are often carried out in the pharmaceutic field by nonisothermal (dynamic) or isothermal thermogravimetric analyses [43–49]. Kinetic studies under isothermal TG conditions are particularly relevant as they are amenable to Arrhenius equation treatment and make possible the estimation of room temperature degradation rate from extrapolation of accelerated, experimental, high-temperature degradation rates [34, 42]. These kinetic studies are particularly useful to provide reliable estimates of the shelf life of pharmaceutical products under various storage and handling conditions [31, 34].

We describe herein the first study on the kinetics of thermal degradation of MnP-based redox-active therapeutics under isothermal and nonisothermal conditions. Additionally, it is worth noting that thermal decomposition kinetic models were evaluated using a state-of-the-art approach, based on the multilayer perceptron (MLP) artificial neural network [48–52]. MnTE-2-PyPCl₅ was chosen as a representative API of the class of Mn(III) 2-N-alkylpyridylporphyrins, currently developed as SOD mimics, biomimetic models, catalytic antioxidants, and redox-active therapeutics.

2. Materials and Methods

MnTE-2-PyPCl₅ and the nonalkylated analogue MnT-2-PyPCl (see Figure 1) were prepared and purified as previously reported [2, 53] and showed characterization features consistent with published data [28, 53–55]. Although these compounds are usually labeled as anhydrous species (i.e., MnTE-2-PyPCl₅), the isolated solids often contain variable amounts of water molecules, depending on sample workup, handling, and storage [28]. In this work, the cationic MnP, MnTE-2-PyPCl₅, contained 8 molecules of water, whereas the neutral MnP, MnT-2-PyPCl, was analyzed as an anhydrous species, according to thermogravimetric data (see below). All samples were investigated by simultaneous thermogravimetric and differential thermal analyses (TG/DTA) under isothermal and nonisothermal conditions. The experimental kinetic designs described below for these two conditions of analyses agree with the International Confederation for Thermal Analysis and Calorimetry (ICTAC) recommendations [45–47].

2.1. Thermal Analysis

TG curves were obtained on a Shimadzu DTG60 thermal analyzer using 2–3 mg samples, accurately weighed in alumina crucibles directly in the thermobalance, under a dynamic atmosphere of synthetic air with a flow rate of 50 mL min^–1.

For the kinetic studies, various TG curves were registered independently using heating rates of 5, 7.5, 10, and 12.5°C min^–1 in the temperature range from 30 to 600°C for nonisothermal experiments. Isothermal experiments were carried out at 158, 160, 162, and 164°C for a period of 60 minutes; in these experiments, the samples were heated from room temperature to the target isothermal temperature with a heating rate of 10°C min^–1.

2.2. Kinetic Study Using the Vyazovkin Isoconversional Method in Nonisothermal TG Data

The solid-state decomposition process follows the general first-order kinetic equation: with as the rate constant following the Arrhenius equation and as the reaction model, with as the conversion degree; , where is the initial sample mass, is the final sample mass, and is the sample mass at time .

Assuming as the heating rate, the temperature integral , and , the general equation (1) can be rewritten as

The isoconversional Vyazovkin [44] method assumes that the reaction model is independent of the heating rate; for this, we have for a given conversion

The frequency factor,, is considered constant for the processes submitted to small variation of the heating rate, so this equation can be treated considering the following optimization:

The temperature integral can be solved assuming the following approximation [56]: with and calculated using the Senum-Yang approximation [56] of the third degree:

The percentage error of the function for the 3^rd rational approximation is about 10^–5 percent [56].

2.3. Kinetic Study Using the MLP Neural Network in Isothermal TG Data

The MLP neural network applied in this study was proposed initially by Sebastião et al. [48–50]. The architecture of this MLP has only one artificial neuron in the input and output layers. The intermediate layer, however, has ten artificial neurons, according to the number of kinetic models considered in the process of the thermal solid decomposition. Table 1 presents the kinetic models studied.

The states of neurons are defined as with being the state of the artificial neuron in the previous layer and being the weights between the neurons and . In this particular type of network, there are also weights called bias, , defined as unity parameters to amplify or reduce the linear correlation of the network [48, 49].

In this proposed architecture of MLP, the weights and the bias were predetermined from the fit of experimental data by each kinetic model in each studied temperature, where the weights are the rate constants and linear coefficient of the kinetic models. These weights do not change in the learning process of the network and can be represented assuming a weight matrix as

Assuming to be the experimental time data for each studied temperature,

In this case, the matrix calculates the state of the artificial neurons in the intermediate layer :

The neurons in the intermediate layer must be activated by an activation function, , to send out information to the output layer. From this, their states are defined by

The activation functions should assume a predetermined value; generally, before calculating the state of neurons; activate the neurons assuming values near to the unity and making sure this is a increasing function , to warranty the energy function optimization [49]. If we chose a linear activation function in the output layer, the network response, given by the state of the neuron in the output layer, is with being the interconnection weight vector of the output layer.

The MLP learning process consists of an energy function optimization, from which only the vector has to be determined [49]. Considering as the experimental data, this energy function is

To solve this equation, the contribution of each kinetic model to describe the experimental data can be calculated by the well-known pseudo-inverse algorithm [49] with .

3. Results and Discussion

3.1. Thermal Decomposition of MnTE-2-PyPCl₅

In our previous studies on the thermal stability of MnTE-2-PyPCl₅ [28], we established that the thermogravimetric events of this Mn-porphyrin under dynamic air were associated with three major processes: (i) dehydration, (ii) N-dealkylation, and (iii) organic matter degradation to yield Mn oxide as the final residue at 900 or 950°C. The N-dealkylation reaction of MnTE-2-PyPCl₅ during the thermogravimetric analyses under air at a heating rate of 10°C min^–1 takes place between 134 and 279°C (see Figure 2). The loss of ethyl chloride (EtCl) was characterized by gas chromatography-mass spectrometry (GC-MS) of the evolved gas (EtCl) and by characterization of the residue at 279°C, which was consistent with MnT-2-PyPCl (see Figure 1) by spectroscopic and chromatographic analyses [28]. Additionally, thermogravimetric analysis of an analytical sample of MnT-2-PyPCl with no N-alkyl moieties (prepared independently [53]) showed no major events in the temperature range associated with EtCl loss in MnTE-2-PyPCl₅ (see Figure 2; see also Figures S1 and S2 and Tables S1 and S2 in Supplementary Materials for complementary data by derivative thermogravimetry (DTG) and differential thermal analysis (DTA)). Whereas dehydration is a reversible process, the N-dealkylation of MnTE-2-PyPCl₅ to yield MnT-2-PyPCl (see Figure 1) represents the first irreversible thermal event that compromises the MnP structural integrity, affecting permanently its catalytic efficiency, lipophilicity, bioavailability, and expected therapeutic outcome [17, 28, 38, 54]. Thus, understanding the kinetics of the MnTE-2-PyPCl₅ thermal N-dealkylation process may prove useful to provide researchers and the pharmaceutic industry with information on handling/storage of MnTE-2-PyPCl₅, shelf life, and usage. Commercially impure samples of Mn(III) 2-N-alkylpyridylporphyrins, containing a mixture of partially N-dealkylated compounds most likely derived from inadequate thermal workup procedures [38, 54], have already clouded some biological conclusions on the in vivo SOD activity of these Mn-porphyrins [54, 57].

Figure 2

Thermogravimetric curve for MnTE-2-PyPCl₅ (red trace, formally MnTE-2-PyPCl₅·8H₂O) and MnT-2-PyPCl (blue trace) under dynamic air at a heating rate of 10°C min^–1. Thermal processes correspond to the following: I—dehydration; II—N-dealkylation; and III—porphyrin ring decomposition to yield Mn oxides. Corresponding data for the nonalkylated sample MnT-2-PyPCl (blue trace) is presented as a control, indicating no N-dealkylation process in the highlighted 134–279°C temperature range. DTG and DTA curves are included as Figures S1 and S2 in Supplementary Materials.

The kinetic studies on the thermal N-dealkylation of MnTE-2-PyPCl₅ were carried out by nonisothermal and isothermal protocols as presented below, observing the recommendations of the International Confederation for Thermal Analysis and Calorimetry (ICTAC) [45, 47]. The specific temperature ranges for the kinetic studies were chosen based on the interval in which only the N-dealkylation process of MnTE-2-PyPCl₅ is prevalent (see Figure 2) [54]. Although thermal kinetic data are usually collected at high temperature under accelerated thermal degradation conditions, the isothermal results may be extrapolated to lower temperatures using the Arrhenius equation, to provide estimates of drug thermal stability under different target temperatures of interest to handling and storage associated with good manufacturing practices [34, 42, 58].

3.2. Nonisothermal Kinetic Analysis

The decomposition process associated with MnTE-2-PyPCl₅ thermal N-dealkylation under nonisothermal conditions was investigated. From this methodology, the kinetic parameters of activation energy () and frequency factor () were determined according to the conversion degree (). The nonlinear isoconversional method proposed by Vyazovkin et al. [45] was used. This method allows an indirect analysis of multistep processes, considering the activation energy as a function of the conversion degree.

Figure 3 shows the nonisothermal experimental data obtained from the four heating rates chosen to follow the MnTE-2-PyPCl₅N-dealkylation process: 5.0, 7.5, 10.0, and 12.5°C min^–1. The curves are not overlapped, and observe the ICTAC recommendations for kinetic studies [45–47]. These curves were treated by the nonlinear Vyazovkin method, and Figure 4 shows the activation energy determined for the thermal decomposition process of MnTE-2-PyPCl₅, as a function of the conversion degree. Figure 4 reveals that the activation energy increases as the conversion proceeds, starting from 75 kJ mol^–1 at the beginning of the process, reaching 100 kJ mol^–1 at , and finishing with values of 125 kJ mol^–1. These high activation energies not only indicate but also are consistent with the observed high thermal stability of the compound [28]. The standard deviations for the Vyazovkin adjustments are presented in Table 2, and their small values warranty that the Vyazovkin method was used properly to treat the MnTE-2-PyPCl₅ thermal N-dealkylation data. It is worth noting that the activation energy represents the energy necessary to initiate the MnTE-2-PyPCl₅ decomposition.

3.3. Isothermal Kinetic Analysis by the MLP Artificial Neural Network

Isothermal decomposition of solids generally occurs at the product-reagent interface [44], being described by kinetic models well established in the literature [48–50]. These models correlate the decomposition fraction, , with time at chosen appropriate temperatures. An adapted multilayer perceptron (MLP) neural network algorithm was developed by our group [48, 49] as a homemade algorithm using the MATLAB language [50, 51]. The algorithm is different from traditional MLP approaches as it does not vary the interconnection weights between the input and intermediate layers. In this MLP approach, only the weights between the intermediate and output layers are optimized, which provides the contribution of each kinetic model in the experimental data description.

In the current study, a total of 12 kinetic models (see Table 1) were investigated simultaneously, out of which 10 models (i.e., D1, D2, D3, D4, R1, R2, R3, Am2, Am4, and Au) were found to describe in a combined manner the thermal decomposition kinetics associated with MnTE-2-PyPCl₅ thermal N-dealkylation reaction. The curves of decomposition fraction, , for MnTE-2-PyPCl₅ isothermal experiments at 158, 160, 162, and 164°C are presented in Figure 5 along with the MLP adjustment considering ten kinetic models for the decomposition process. As shown in Figure 5, the residual error of the MLP adjustment in all cases is about 10^–4, attesting the robustness of the method.

The MLP neural network approach is exceptionally accurate compared to the adjustment of each individual kinetic model separately. Figure 6 presents the residual error of each kinetic model considered individually to fit the experimental data. It is worth noting that fitting of experimental data with various kinetic models resulted in comparable residual errors, many of which are of the same order of magnitude (see Figure 6). This result strongly suggests that the N-dealkylation of MnTE-2-PyPCl₅ via a thermal decomposition process is better understood as an overlapping contribution of many mechanisms combined [48, 49]; for this case, the R1, R2, Am2, and Am4 mechanisms presented smaller residual error to fit the experimental data. Nonetheless, the contraction models R1 and R2 explain the experimental data with more accuracy. The R2 model assumes the nucleation occurring rapidly on the surface of the crystal, which presents a cylinder shape. In this kinetic model, that accounts for a contracting area; the radius contraction follows , where is the radius at time , is the initial radius, and is the rate constant, as schematically represented in Figure 7. The generalized models, which encompass R1 and R2, are mathematical functions that describe reactions taking place in the boundary phase, with diffusion processes being extremely fast, which prevents an adequate interaction of reactants in the interface [41, 59]. These phase boundary-controlled reaction processes are, thus, controlled by the rearrangement of the reactants in the limitrophe phase. The models are known as geometrical contraction models, but R1 is similar to the ones usually applied to describe the reaction order mechanisms in a homogeneous system, following a zero-order reaction rate.

In the MLP artificial neutral network approach, ten kinetic models (i.e., D1, D2, D3, D4, R1, R2, R3, Am2, Am4, and Au, see Table 1) were considered simultaneously in the intermediate layer, with the matrix (equation (8)) determined by the rate constant () and linear coefficient () of kinetic models by the fitting experimental data of each isothermal curve. The contribution of the kinetic models to the MnTE-2-PyPCl₅ isothermal decomposition process is presented in Figure 8, which reveals that for all studied temperatures (158, 160, 162, and 164°C), the most significant contributions were those associated with the contraction models R1 and R2. Thus, the contraction models R1 and R2 showed both smaller error and greater contribution in describing the phenomenon of MnTE-2-PyPCl₅ thermal decomposition.

The rate constants determined by the experimental data adjustment into the kinetic models via the MLP neural network approach ( matrix, equation (8)) were used to calculate the activation energy of MnTE-2-PyPCl₅ thermal decomposition. The MLP-derived values shown in Figure 9 are comparable to the activation energies calculated using the Vyazovkin method (see Figures 4 and 9). This agreement with the nonisothermal result indicates that the decomposition process is not affected by the experimental condition (i.e., isothermal or nonisothermal protocol) and, again, the MLP values of activation energy are consistent with the overall stability of MnTE-2-PyPCl₅.

MnTE-2-PyPCl₅ is often stored as a nonformulated solid or as aqueous solution formulations [9], [10, 17]. Nonformulated solid MnTE-2-PyPCl₅ has often been stored at room temperature, presumably between 15 and 35°C [9, 10, 28]. Thus, considering the activation energy values and rate constants determined at high temperature, the kinetic parameters at 25°C were estimated, using the Arrhenius equation. These kinetic data also allowed the estimation of the shelf life value for MnTE-2-PyPCl₅ (i.e., the time in which the initial MnP concentration decays by 10%) at 25 and 158°C (see Table 3). The value is an important parameter that is recommended for estimating the API shelf life [34, 60]. As the model R1 showed smaller error and greater contribution to the phenomenon of thermal decomposition of the material, the value found by this model is the most adequate to describe the behavior of MnTE-2-PyPCl₅ over time. Therefore, it is expected that it would take nearly 17 years for solid MnTE-2-PyPCl₅ to reach a 10% N-dealkylation at 25° C, which is consistent with the high stability empirically observed for this compound on daily routine handling and storage in the laboratory [54]. Of note, a solid sample of high-purity MnTE-2-PyPCl₅ stored in a closed vial at room temperature in the dark for 7 years in our laboratories showed no noticeable signs of N-dealkylation when analyzed by thin-layer chromatography (data not shown). As shelf life values may be significantly affected by storage and drug formulations (including the nature of excipients) [34, 52, 60], the calculated value for MnTE-2-PyPCl₅ is valid if the net solid material is kept in ideal conditions of humidity. As noted, MnTE-2-PyPCl₅ is a hydratable molecule. The presence of humidity can trigger hydrolytic processes. High humidity generates absorption of the excess moisture in the air, which can be just as destructive as environments with low humidity. A humidity excess can compromise potency and effectiveness, leading to degradation or even toxicity in some products. It may promote the growth of microorganisms, compromising their biological integrity. The International Conference on Harmonization (ICH) in the topic Q1 A(R2) deals with the stability testing of new drugs and products, delimiting good practices of manipulation and storage concerning temperature and humidity parameters. Those variables take into account the country climate zone classification. MnTE-2-PyPCl₅ must be stored in a hermetically sealed flask and kept at a storage temperature of and relative humidity of whereas Brazil belongs to climate zone IV [61, 62]. It is worth noting that these data drive further studies on MnTE-2-PyPCl₅ formulations and stability.

4. Conclusions

The first study on the thermal stability kinetics of Mn(III) 2-N-alkylpyridylprophyrin was carried out with the prototypical MnTE-2-PyPCl₅ compound using thermogravimetry (TG) under nonisothermal and isothermal conditions. The new multilayer perceptron (MLP) artificial neural network approach allowed the simultaneous study of ten solid-state kinetic models and showed that N-dealkylation associated with MnTE-2-PyPCl₅ degradation is better explained by a combination of various mechanisms, with major contributions from the contraction models R1 and R2. The calculated activation energy values from isothermal and nonisothermal data agreed with one another and were consistent with the observed high thermal stability of MnTE-2-PyPCl₅ in the solid state. The shelf life value at 25°C estimated from the isothermal decomposition data was approximately 17 years. These results contribute to the development of this class of redox-active therapeutics and SOD mimics and provide supporting data to protocols on purification, handling, storage, formulation, expiration date, and general use of these compounds.

Additionally, these MnTE-2-PyPCl₅ results not only set the grounds for studying other Mn(III) porphyrin-based SOD mimics and redox-active therapeutics but also represent a first approximation to the thermal stability kinetic parameters of the chemically related Zn(II) porphyrin counterpart, ZnTE-2-PyPCl₄, and its Zn(II) 2-N-alkylpyridylporphyrin analogues, which are being actively pursued as efficient and selective photosensitizers for antimicrobial photodynamic inhibition (aPDI) [63–67].

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

Acknowledgments

C.G.C.M and B.C.L.A acknowledge the PhD scholarships by CNPq and CAPES, respectively. We thank Dr. Victor Hugo A. Pinto and Dr. Jacqueline C. Bueno-Janice for the analyses of a 7-year-old sample of MnTE-2-PyPCl₅. J.S.R dedicates this paper to the memory of Dr. Margaret Tome who greatly advanced our current understanding on the biological modes of action of MnTE-2-PyPCl₅ and MnP-based redox-active therapeutics. This study was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Financiadora de Estudos e Projetos (FINEP), and Universidade Federal da Paraíba (UFPB).

Supplementary Materials

The thermogravimetry (TG), derivative thermogravimetry (DTG), and differential thermal analyses (DTA) were provided for both MnTE-2-PyPCl₅ and MnT-2-PyPCl samples. (Supplementary Materials)

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Copyright © 2021 Clarissa G. C. Maia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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