Integrative Network Analysis Revealed Genetic Impact of Pyruvate Kinase L/R on Hepatocyte Proliferation and Graft Survival after Liver Transplantation

Figure 4

Comprehensive analysis on nontargeted metabolomic results in grafts for transplantation classified by PK variations. (a) Heatmap for correlation between interconnection between PKLR/PKM genes and clinical factors in LT; frame in represented the correlation with statistical significance (). (b) Correlation analysis between log-transformed PKLR and PKM expression in grafts for LT. (c) Analysis on grafts’ survival categorized by PKLR/PKM expression. (d) Analysis on patients’ survival categorized by PKLR/PKM expression. (e) Distribution of EAD occurrence in patients categorized by PKLR/PKM expression. (f) Distribution of PNF occurrence in patients categorized by PKLR/PKM expression. (g) PCA in patients categorized by PKLR expression, dots in blue represented the samples with higher PKLR, and the dots in green represented the samples with lower PKLR. (h) Heatmap for clusters of metabolites significantly associated with PKLR expression. (i) Volcano plot on visualization of both FC and significance for each metabolite compared between higher and lower PKLR expression, red dots represented significantly higher metabolites () in grafts with higher PKLR, and green dots represented significantly lower metabolites () in grafts with higher PKLR. (j) Correlation heatmap for the top 20 metabolites associated with PKLR expression. (k) Overlap between significant metabolites in cells and grafts by KEGG identification. (l) FC of C00157 and C04230 categorized by PKM/PKLR expression. (m) Scatter plot for correlation between log-transformed PKLR and C00157 expression. (n) Scatter plot for correlation between log-transformed PKLR and C04230 expression. (o) Scatter plot for correlation between log-transformed PKM and C00157 expression. (p) Scatter plot for correlation between log-transformed PKM and C04230 expression. Abbreviations: EAD: early allograft dysfunction; FC: fold change; LT: liver transplantation; PCA: principal component analysis; PNF: primary nonfunction.

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