Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

Figure 2

I-R induced neuronal death, which could be rescued by TRPM2 deficiency. (a) Primary cultured cortical neurons were subjected to 1 h of OGD followed by 3 or 6 h of reperfusion (OGD-R 3 h and OGD-R 6 h) at DIV9. The cleaved Casp3, Casp3, and actin levels in cultured neurons were determined by Western blot. (b) Semiquantitative analysis showed the ratio of the cleaved Casp3 and Casp3 band intensities. (c) Mice were subjected to middle cerebral artery occlusion for 1.5 h, and reperfusion was allowed by removing the monofilament suture. Animals were euthanized 24 h after tMCAO, and representative TTC-stained brain slices from each group are shown. (d) Infarct volumes were determined by TTC staining in the bar charts (, ). The neurological deficit scores of each group are presented. Data was quantified from 3–4 independent experiments. ; ; ; ne-way ANOVA with post hoc Tukey test.

(a)

(b)

(c)

(d)