Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

Figure 3

Inhibition of autophagy abolishes the protection of TRPM2 deficiency in I-R injury. Mice were subjected to middle cerebral artery occlusion for 1.5 h, and reperfusion was allowed for 24 h with (a) LY294002 (5 mg/kg) or (c) 3-MA (7.5 μg) by removing the monofilament suture. Animals were euthanized 24 h after tMCAO, and representative TTC-stained brain slices from each group are shown. Infarct volume and neurological deficit score were presented in the bar charts with treatment of (b) LY294002 or (d) 3MA (, ). Unpaired Student’s -test.

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