Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

Figure 4

TRPM2 deficiency promotes autophagy induction under OGD-R in primary cortical neurons. (a) Primary cultured neurons from WT and TRPM2 KO mice were subjected to 2 h of OGD plus 1 or 3 h of reperfusion in DIV9. Autophagosome was observed by electron microscopy. Autophagosome was labelled as A and nucleus as N. (b) Primary cultured neurons transfected with mRFP-GFP-LC3 were subjected to 1.5 h of OGD plus 1 or 3 h of reperfusion (OGD-R 1 h and OGD-R 3 h) in DIV9. Representative z-stack confocal images showing the colocalization of mRFP-LC3 with GFP-LC3 induced by OGD-R. (c) Columns represent number of mRFP-GFP-LC3 puncta per cell (). Scale bars: 10 μm. Data was quantified from 3–4 independent experiments. ; one-way ANOVA with post hoc Tukey test.

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