Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

Figure 5

TRPM2 deficiency promotes autophagy induction during I-R injury in vivo. (a) WT and TRPM2-KO mice were subjected to sham or MCAO for 1.5 h followed by 1, 3, or 6 h of reperfusion. Then, brain slices were mounted, and confocal images were taken. Representative images of cells expressing AAV-mRFP-GFP-LC3 of brain slices from WT and TRPM2-KO mice are shown. (b) Summary graph showing the quantification of the number for overlapped puncta, as determined by colocalization of all cells from the experiments shown in (a). Columns represent number of mRFP-GFP-LC3 puncta per cell (). Scale bars: 5 μm. Data was quantified from 3–4 independent experiments. ; one-way ANOVA with post hoc Tukey test.

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