Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

Figure 6

TRPM2 deficiency-promoted autophagy is mediated by the AMPK/mTOR pathway in I-R injury. (a) WT and TRPM2-KO mice were subjected to sham or MCAO for 1.5 h, and reperfusion was allowed for 6 h by removing the monofilament suture. Then, the mice were euthanized, and ischemic penumbra was collected for immunoblot. 3 μg of protein was sequentially immunoblotted with antibodies against AMPK, phosphorylated-AMPK (p-AMPK), mTOR and phosphorylated-mTOR (p-mTOR), and β-actin. Semiquantitative analysis of (b) p-AMPK/AMPK and (c) p-mTOR/mTOR is shown. Data was quantified as from 3-4 independent experiments. ; one-way ANOVA with post hoc Tukey test.

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