Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy

<div>Inhibition of TRPM2 or mTOR enhanced autophagy induction in I-R injury. (a) SH-SY5Y cells overexpressing mRFP-GFP-LC3 were treated with OGD for 6 h and then reperfusion for 6 h with normal medium. 30 <i>μ</i> M ACA, 5mM NAC, or 1 <i>μ</i>M Torin1 was added during reperfusion. Scale bar: 5 <i>μ</i>m. (b) The average numbers of red LC3 puncta per cell in each condition were quantified. 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d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>;</span> one-way ANOVA with post hoc Tukey test.</div>

Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy 