Trigonelline Extends the Lifespan of C. Elegans and Delays the Progression of Age-Related Diseases by Activating AMPK, DAF-16, and HSF-1

Figure 1

Trigonelline can prolong the lifespan and improve the health of C. elegans (a) Chemical structure of trigonelline; (b) Survival curves of wild-type N2 worms raised at 20°C on NGM plates containing either no trigonelline or 25, 50, 100, and 200 μM of trigonelline; (c) Survival curves of wild-type N2 worms treated from hatching and raised at 20°C on NGM plates containing either no trigonelline or 50 μM of trigonelline (p <0.001, log-rank test), Statistical details and repeats of these experiments were summarized in Table S1; (d) Body bending of N2 nematode treated with 50 μM of trigonelline for 5 days and 10 days. Figure shows the mean value of three independent experiments, SEM is represented by error line. represents p <0.001, calculated by two-tailed t-test. Statistical details and repeats of these experiments were summarized in Table S3; (e) Analysis of lipofuscin in Treatment of N2 nematodes by 50 of μM trigonelline on day 10. Relative Fluorescence Intensity was calculated by Image J. Figure shows the mean value of three independent experiments, SEM is represented by error line. represents p <0.001, calculated by two-tailed t-test. Statistical details and repeats of these experiments were summarized in Table S2.

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