Trigonelline Extends the Lifespan of C. Elegans and Delays the Progression of Age-Related Diseases by Activating AMPK, DAF-16, and HSF-1

Figure 2

Trigonelline can enhance the oxidative stress resistance of C. elegans. The survival percentage of wild-type worms cultured with 20 mM of paraquat under treatment with 50 μM of trigonelline (p <0.001, log-rank test). Statistical details and repeats of these experiments were summarized in Table S6; (B-C) The quantification of fluorescence intensity of SOD-3::GFP in CF1553 and GST-4::GFP in CL2166. Trigonelline significantly increased the expression of SOD-3 and GST-4, Fluorescence intensity was calculated by Image J. The bar chart shows the mean value of three independently repeated experiments, and the error line represents SEM. represents p <0.001, calculated by two-tailed t-test. Statistical details and repeats of these experiments were summarized in Table S5; (D) ROS content detection of wild type N2 treated with 50 μM of trigonelline, while 10 mM of paraquat and 2 mM of NAC were used as negative control and positive control, respectively. Statistical details and repeats of these experiments were summarized in Table S7.

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