Trigonelline Extends the Lifespan of C. Elegans and Delays the Progression of Age-Related Diseases by Activating AMPK, DAF-16, and HSF-1

Figure 3

Trigonelline can enhance the ability of nematode to resist heat and pathogenic stresses (a) The survival percentage of wild-type worms treated with or without 50 μM of trigonelline at 35°C (p <0.001), Statistic details and repeats of these experiments were summarized in Table S6; (B-D) The quantification of fluorescence intensity of HSP-6::GFP in SJ4100, HSP-4::GFP in SJ4005,HSP-60::GFP, and in SJ4058. Trigonelline significantly increased the expression of HSP-4 and HSP-6, Fluorescence intensity was calculated by Image J. The bar chart shows the mean value of three independently repeated experiments, and the error line represents SEM. represents p <0.001, calculated by two-tailed t-test. Statistical details and repeats of these experiments were summarized in Table S5; (E) The survival percentage of wild-type worms cultured with Pseudomonas aeruginosa under treatment with 50 μM of trigonelline (p <0.001, log-rank test). Statistical details and repeats of these experiments were summarized in Table S6; (F) Relative Expression of Immune Genes in L4 Wild-type Worm (N2) Treated with 50 μM of trigonelline for 24 h. Statistical details and repeats of these experiments were summarized in Table S9.

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