Peroxisome Deficiency Dysregulates Fatty Acid Oxidization and Exacerbates Lipotoxicity in β Cells

Figure 1

Knockdown (KD) of Pex14 causes defective expression of peroxins implicated in peroxisome biogenesis and degradation. (a, b) Knockdown efficiency of INS-1 cells transfected with si-Pex14 or si-NC was examined by q-PCR (a) and western blotting (b). (c) Representative IF photomicrographs of Pex14 in INS-1 cells (×800 magnification, scale bar 100 μm). (d, e) The expression of peroxins and the transcription factors that regulate peroxins was detected by western blot (d) or q-PCR (e). -4; error bars indicate SD. , vs. the blank or si-NC group.

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