Research Article
Peroxisome Deficiency Dysregulates Fatty Acid Oxidization and Exacerbates Lipotoxicity in β Cells
Figure 3
Knockdown of Pex14 aggravated fatty acid-induced intracellular ROS accumulation. (a, b) Antioxidant factors were detected by immunoblot assay (a) and q-PCR (b); , vs. the blank or si-NC group. (c) IF showed the decreased expression and location of catalase. (d) Intracellular ROS were detected by flow cytometry in INS-1 cells treated with LA or PA using the probes DCFH-DA and the average -mean values were calculated. NT: no treatment. , vs. the NT or LA group. #, vs. the si-NC with or without LA/PA group.
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