Peroxisome Deficiency Dysregulates Fatty Acid Oxidization and Exacerbates Lipotoxicity in β Cells

Figure 5

Knockdown of Pex14 aggravated fatty acid-induced apoptosis. (a, b) Cell Counting kit-8 assay was used to evaluate the effect of Pex14 KD on INS-1 cell viability with or without LA (a) or PA (b) treatment. , vs. the time-matched blank or si-NC group. ^#, vs. the si-NC with or without LA/PA group. (c) Cell apoptosis was assessed by flow cytometry with an Annexin V-PI kit. , vs. the NT or LA group. ^#, vs. the si-NC with or without LA/PA group. (d) The apoptosis regulators c-caspase3 were detected by immunoblot assay. (e) q-PCR detect the transcription of Pex14, Abcd3, and Acaa1a in Pex14 KD cells with PA treatment. , vs. the blank or si-NC group.

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