Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress

<div>Rap1GAP knockdown inhibited Ang II-induced ROS production and apoptosis. (a) Phosphorylated protein and total protein expression of AMPK, AKT, mTOR, and p70s6K in Rap1GAP knockdown vs. control cardiomyocytes. (b) ROS production in Rap1GAP knockdown and intact cardiomyocytes evaluated using DHE fluorescent dyes. Scale bar: 50 <i>μ</i>m. (c) Flow cytometric analysis for NRCM apoptosis stained with 7AAD and Annexin V-PE. 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(d) TUNEL staining for cardiomyocyte apoptosis assay. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress 