Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress

Figure 8

Reduced autophagy induced by Rap1GAP is associated with ROS production. (a) Western blot analysis for the expression of LC3B and p62 in Rap1GAP overexpressing cardiomyocytes treated with rapamycin. (b) ROS production in Rap1GAP overexpressing cardiomyocytes after intervention with rapamycin, determined using DHE fluorescent dye staining. Scale bar: 50 μm. (c) Western blot analysis for LC3B and p62 in Rap1GAP knockdown cardiomyocytes treated with 3-Methyladenine. (d) ROS production in Rap1GAP knockdown cardiomyocytes treated with 3-Methyladenine, determined using DHE fluorescent dye staining. Scale bar: 50 μm. and . Results are the of three independent experiments ().

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