Inhibition of the Keap1/Nrf2 Signaling Pathway Significantly Promotes the Progression of Type 1 Diabetes Mellitus

Figure 6

ALX significantly inhibited the Keap1/Nrf2 signaling pathway in pancreatic β-cells. (a) The protein levels of Keap1, Nrf2, HO-1, and NQO1 in whole-cell lysates after treatment with vehicle and ALX (1.5, 3, and 6 mM) for 24 h were detected by Western blot analysis. (b) The nuclear Nrf2 protein levels were also detected after the same treatment. (c) Representative confocal images (scale bar: 50 μm) of MIN6 cells subjected to the same treatments and stained for Nrf2 (red) and with DAPI (blue). The data represent the (). , , and compared with the control group.

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