ASC Speck Formation after Inflammasome Activation in Primary Human Keratinocytes

Figure 1

(a) UVB irradiation leads to the formation of ASC specks in primary human keratinocytes. Primary human keratinocytes were irradiated with UVB (50 mJ/cm²). Exposure to UVB light leads to the formation of nuclear ASC “specks”. (b) zVAD does not inhibit ASC “speck” formation, but suppresses IL-1 secretion. Keratinocytes were treated with 20 μM zVAD for 1 h and were irradiated with UVB (50 mJ/cm²). ASC (red), F-actin (green), and DAPI (dark blue). IL-1α and IL-1β were measured in cell-free supernatants using ELISA. Data represent at least 3 independent experiments and are presented as the . Statistics: one-way ANOVA statistical test. Symbols for values used in the figures. , , , and . NS: not significant. Magnification: ×43.

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