ASC Speck Formation after Inflammasome Activation in Primary Human Keratinocytes

Figure 7

(a) IFI16 interacts with ASC after UVB sensing in HaCaT cells. HaCaT cells were irradiated with UVB. After 0.5 h, 2 h, and 5 h, the interaction of ASC with IFI16 was observed using immunoprecipitation. ASC interacts with IFI16 following UVB radiation with the maximum interaction occurring 2 hours after stimulation. (b) p53 and IFI16 translocation to the nucleus was impaired in ASC knockdown HaCaT cells. Cells were knocked down with ASC and then irradiated with UVB. The translocation of p53 and IFI16 was absent in HaCaT cells that have been knocked down with ASC.

(a)

(b)