Research Article
ASC Speck Formation after Inflammasome Activation in Primary Human Keratinocytes
Table 1
Oligonucleotide primers and LightCycler hybridization probes.
| Target gene | Accession no. | Primer | Sequence | Purpose |
| β-Actin | P60709 | ACTIN F | AGCCTCGCCTTTGCCGA | qPCR | ACTIN R | CTGGTGCCTGGGGCG | qPCR | ACTIN TM | 6FAM-CCGCCGCCCGTCCACACCCGCC—BBQ | Hybridization probe | NLRP1 | Q9C000 | NLRP1 F | CACTTTATATGGGCTGTCGTTACA | qPCR | NLRP1 S | GGGTCTGGTTCAGGGATGC | qPCR | NLRP1 A | CCAACGTAGAACTCCGAGAACA | Hybridization probe | NLRP1 R | CTCATCTTTCTTGTCTTTCACTTGC | | NLRP1 P | 6FAM-CTCCAGGGCTTCGATAGCAGAGCT—BBQ | | NLRP3 | Q1JQ87 | NLRP3 F | CACTTCTGACCTCCAGCCA | qPCR | NLRP3 S | CAACAATGACCTGGGCGA | qPCR | NLRP3 A | TCTTCTTGAAGTGTTTCTAACGCA | Hybridization probe | NLRP3 R | AGGCTCAAAGACGACGGT | | NLRP3 | 6FAM-CTGAAACAGCAGAGCTGCCTCCTG—BBQ | |
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