ASC Speck Formation after Inflammasome Activation in Primary Human Keratinocytes

Table 1

Oligonucleotide primers and LightCycler hybridization probes.

Target gene	Accession no.	Primer	Sequence	Purpose
β-Actin	P60709	ACTIN F	AGCCTCGCCTTTGCCGA	qPCR
		ACTIN R	CTGGTGCCTGGGGCG	qPCR
		ACTIN TM	6FAM-CCGCCGCCCGTCCACACCCGCC—BBQ	Hybridization probe
NLRP1	Q9C000	NLRP1 F	CACTTTATATGGGCTGTCGTTACA	qPCR
		NLRP1 S	GGGTCTGGTTCAGGGATGC	qPCR
		NLRP1 A	CCAACGTAGAACTCCGAGAACA	Hybridization probe
		NLRP1 R	CTCATCTTTCTTGTCTTTCACTTGC
		NLRP1 P	6FAM-CTCCAGGGCTTCGATAGCAGAGCT—BBQ
NLRP3	Q1JQ87	NLRP3 F	CACTTCTGACCTCCAGCCA	qPCR
		NLRP3 S	CAACAATGACCTGGGCGA	qPCR
		NLRP3 A	TCTTCTTGAAGTGTTTCTAACGCA	Hybridization probe
		NLRP3 R	AGGCTCAAAGACGACGGT
		NLRP3	6FAM-CTGAAACAGCAGAGCTGCCTCCTG—BBQ