Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 2

VB 12 reduces necrosis and inflammatory response of pancreatic acinar cells by activating CBS. (a) Representative images showing Hoechst 33342 (blue) and PI (red) staining in pancreatic acinar cells stimulated with NaT (5 mM) in the absence or presence of VB 12 (60,100 nM). (b) The necrosis rate was quantified by image J. (c, d) The levels of TNF-α and IL-1β in the lysate of acinar cells were detected by ELISA kit. (e) The expression of CBS protein in acinar cells was detected by Western blot. (f) The GSH/GSSG ratio in acinar cells was detected by assay kit. Data are expressed as , experiments were repeated more than three times. vs. Con group, ^## vs. NaT group, ^### vs. NaT group, ^∆∆ vs. VB 12 (60 nM) group, ^∆∆∆ vs. VB 12 (60 nM) group. Con: control group; NaT: acute pancreatitis group.

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