Vitamin B₁₂ Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway

Figure 3

VB 12 inhibits oxidative stress in acinar cells by activating CBS and SIRT1. (a, b) Fresh isolated acinar cells were incubated with DCFH-DA (1 μM) in dark for 20 minutes, washed twice, and then, incubated with NaT (5 mM) alone or combined with VB 12 (60 nM, 100 nM) for 30 minutes. Fluorescence microscopy was used to capture the fluorescence intensity of ROS in cells and quantify the level of total ROS in cells. (c–e) Western blot analysis of protein levels of p-p38, p38, and Nrf2 in acinar cells treated with NaT, NaT + VB 12 (60 nM), and NaT + VB 12 (100 nM). (f–h) The expression of SIRT1 and PGC1-α protein in acinar cell lysate were analyzed. Data are expressed as ; experiments were repeated more than three times. vs. Con group, vs. Con group, ^# vs. NaT group,^## vs. NaT group, ^∆ vs. VB 12 (60 nM) group, ^∆∆ vs. VB 12 (60 nM) group. Con: control group; NaT: acute pancreatitis group.

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