Role of Calcium Signaling Pathway-Related Gene Regulatory Networks in Ischemic Stroke Based on Multiple WGCNA and Single-Cell Analysis

Figure 2

Expression of hub genes in the mouse brain vasculature. (a, b) UMAP analysis showing the results of descending clustering and annotation of cell subpopulations (endothelial cells, fibroblasts, oligodendrocytes, and microglia) in cells of the mouse cerebrovascular system. (c) ANOVA analysis showing the variation of gene expression in the mouse cerebrovascular system cells. Variable genes are indicated by red dots according to selected thresholds, and nonvariable genes are indicated by black dots. The relevant hub genes (Bcl10, Bcl2A1a, Bcl2A1b, Bcl2A1c, Bcl2A1d, Bre, Dynll2, and Ptgs2) are indicated in the figure. (d) Relative expression of related hub genes in various cell subpopulations. (e) A heat map showing the relative expression of each molecule in cell subpopulations and cell clusters. The relative expression profiles of marker genes associated with each cell subpopulation are known.

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