Trophoblasts Modulate the Ca²⁺ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor

Figure 4

The transfer of exosomal miR-25-3p into HMSMs. The sEVs derived from HTR-8/SVneo cells were observed under transmission electron microscopy (TEM) (a). The sEVs were analyzed by Nanoparticle Tracking Analysis (NTA) (b). The sEV markers were detected by western blot (c). The HMSMs were incubated with Dil-labeled sEVs for 24 h, and the fluorescence was observed (d). Red: Dil, blue: DAPI. The HTR-8/SVneo cells were transfected with the Cy3-conjugated miR-25-3p mimic or Cy3 dye only. Then, the cells were cocultured with HMSMs for 12 h, and the fluorescence of HMSMs was detected (e). Red: Cy3, blue: DAPI. The expression of miR-25-3p in HMSMs was measured by real-time PCR (f). HMSMs: human myometrial smooth muscle cells.

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