Trophoblasts Modulate the Ca²⁺ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor

Figure 7

The inhibition of miR-25-3p in HMSMs reversed exosome-induced Ca²⁺ oscillation and contraction. The HMSMs were transfected with miR-25-3p antagomir before being applied with the sEVs derived from HTR-8/SVneo (the HTR-8/SVneo cells have been transfected with miR-25-3p agomir and treated with LPS), and the expression of CACNA1H and ATP2A2 in the HMSMs was determined by real-time PCR (a). The expression of Cav3.2 and SERCA2a in HMSMs was measured by western blot (b). The cells were preincubated with the indicated sEVs; then, the Ca²⁺ transients of HMSMs were measured in the presence of cyclopiazonic acid (CPA) (c). The cells were preincubated with the indicated sEVs; then, the Ca²⁺ transients of HMSMs were measured in the presence of oxytocin (d). The cells were preincubated with the indicated sEVs; then, the contraction of HMSMs was detected by the collagen matrix contraction assay (e).

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