Autophagic Flux Unleashes GATA4-NF-κB Axis to Promote Antioxidant Defense-Dependent Survival of Colorectal Cancer Cells under Chronic Acidosis

Figure 2

NF-κB is upregulated in CRC-AA cells. (a) The expression of NRF2 in CRC-AA and their parental cells was measured by Western blotting. (b) Levels of p-p65 and p65 determined by Western blotting. (c) Western blot analysis of p-p65 in cytoplasmic and nucleus components, respectively. GAPDH and histone H3 were used as loading controls for the cytoplasmic and nuclear proteins, respectively. (d) Immunofluorescence staining of NF-κB/p65 in CRC and CRC-AA cells. (e) mRNA levels of cytokines measured by quantitative real-time PCR. Data shown were representative of three independent experiments. ; ; .

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