Autophagic Flux Unleashes GATA4-NF-κB Axis to Promote Antioxidant Defense-Dependent Survival of Colorectal Cancer Cells under Chronic Acidosis

Figure 5

Upregulation of NF-κB is driven by GATA4 in CRC-AA cells. (a) Expression levels of GATA4 determined by Western blotting. (b) Immunoblotting of endogenous GATA4 immunoprecipitated by p62 in CRC cells treated with chloroquine (25 μM) for 24 h under pH 7.4 and pH 6.5 medium, respectively. (c) Knockdown of GATA4 by siRNA for 48 h, p-p65, p65, and GATA4 protein levels were determined by Western blotting. (d) DCF intensity in CRC and CRC-AA cells transfected with GATA4 siRNA for 24 h. (e) Apoptosis was measured by flow cytometry in cells transfected with GATA4 siRNA for 48 h. (f) NAC attenuates GATA4 siRNA-induced apoptosis. Cells were transfected with GATA4 siRNA for 48 h in the presence of the NAC (10 mM). ; ; ns, .

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