TNF-α Induces Neutrophil Apoptosis Delay and Promotes Intestinal Ischemia-Reperfusion-Induced Lung Injury through Activating JNK/FoxO3a Pathway

Figure 4

TNF-α inhibitor inhibits polymorphonuclear neutrophil (PMN) infiltration of lung tissue. A mouse model of IIR injury was constructed and treated with TNF-α inhibitor lenalidomide, followed by using naphthol esterase staining to detect PMN infiltration of lung tissue (a); the expression level of MPO, a PMN marker, in lung tissue was detected using ELISA (b); and the number of Ly6G-positive PMNs in lung tissue was detected (c) and counted (d) using immunohistochemical staining. Each bar represents the ( per group). , , one-way ANOVA with Tukey test. Sham: sham-operated group; IIR: intestinal ischemia-reperfusion model group; lenalidomide: intravenous administration of lenalidomide (10 mg/kg) alone; IIR+lenalidomide: immediate intravenous administration of lenalidomide (10 mg/kg) at the onset of IIR.

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