TNF-α Induces Neutrophil Apoptosis Delay and Promotes Intestinal Ischemia-Reperfusion-Induced Lung Injury through Activating JNK/FoxO3a Pathway

Figure 5

TNF-α affects polymorphonuclear neutrophil (PMN) apoptosis through the JNK/FoxO3a pathway. TNF-α stimulated PMNs to mimic the in vivo inflammatory environment, and the level of PMN injury was detected using the CCK-8 method (a); the rate of PMN apoptosis was detected using flow cytometry (b); the levels of FoxO3a, JNK, and Bcl-2 were detected using western blotting (c). The data are presented as the , independent experiments. , , one-way ANOVA with Tukey test. Con: control group; TNF-α: a model group in which PMNs were stimulated using TNF-α (100 ng/mL) for 12 h; siRNA-scramble+TNF-α: the negative control group; siRNA-FoxO3a+TNF-α: PMNs treated with TNF-α (100 ng/mL) for 12 h at 48 h after transfection with siRNA-FoxO3a; SP600125: PMNs treated with the antiapoptotic agent SP600125 (20 μM) alone; SP600125+TNF-α: PMNs treated with TNF-α (100 ng/mL) and SP600125 (20 μM) for 12 h; PBS: phosphate-buffered saline.

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